Trifluoroacetic Acid
Trifluoroacetic acid is a corrosive organofluorine compound that is structurally analogous to, but stronger than, acetic acid. Available in various quantities and reagent grades, it is used in NMR spectroscopy, mass spectrometry, organic synthesis, etc.
Almost 100,000-fold more acidic than acetic acid, TFA is widely used in organic chemistry. TFA is used as a reagent in organic synthesis because of its properties: volatility, organic solvent solubility, and acidic strength. It is less oxidizing than sulfuric acid and is more readily available in its anhydrous form than some other acids . Other features include:
- Used in acid-catalyzed reactions, especially peptide synthesis (to cleave esters)
- Dissolves protein when mixed with liquid SO2
- Removes t-butyl derived side-chain protecting groups in Fmoc peptide synthesis
- Can remove the t-butoxycarbonyl protecting group in organic synthesis
- At low concentrations, acts as an ion-pairing agent for peptides and small proteins in organic compound liquid chromatography
- For acid-stable materials, can be a solvent for NMR spectroscopy
- Acts as a calibrant in mass spectrometry
- Used to produce trifluoroacetate salts
- An ingredient in adhesives, sealants, paints, and coatings
When TFA combines with bases and metals, especially light metals, a strong exothermic reaction occurs. When mixed with lithium aluminum hydride (LAH), the reaction is explosive.
Although nonflammable, TFA is corrosive to skin, eyes, and mucous membranes and requires careful use and handling. It is harmful when inhaled, causes severe skin burns and eye damage, and is toxic to aquatic organisms at even low concentrations.
TFA is also a product of the metabolic breakdown of the anesthetic agent halothane. It is the suspected cause of halothane-induced hepatitis.
Medchemexpress LLC DLPLTFGGGTK TFA | MFCD34187350 | 98.1% | 1219.26 | 1 MG
DLPLTFGGGTK TFA is a synthetic peptide, presented as a white to off-white solid. It is a surrogate peptide for pembrolizumab identification and has been tested to comply with given specifications. This high-purity product (98.11%) is intended for research use only.
- White to off-white solid
- Surrogate peptide for pembrolizumab identification
- High purity of 98.11%
- Molecular weight of 1219.26
- Intended for research use
Medchemexpress LLC Lys-coenzyme A trifluoroacetate | 98.5% | 1108.82 g·mol⁻1 | C33H54F3N10O21P3S | 5 MG
Lys-CoA TFA is a bisubstrate-mimic inhibitor of the p300 histone acetyltransferase (HAT). It inhibits p300-dependent acetylation (reported IC50 = 50-500 nM) with >100-fold selectivity over PCAF and is intended for use in biochemical and cell-based studies probing p300-mediated acetylation.
- Selective p300 histone acetyltransferase (HAT) inhibitor (IC50 = 50-500 nM)
- Greater than 100-fold selectivity versus PCAF
- High purity (98.5%) suitable for research applications
- Molecular formula C33H54F3N10O21P3S
- Molecular weight 1108.82 g·mol⁻1
- Supplied as a small-quantity solid for biochemical assays and cell studies
Medchemexpress LLC Ac-VDVAD-CHO (TFA) | 00-00-0 | 99.2% | 657.59 g·mol⁻¹ | C25H38F3N5O12 | 5 MG
Ac-VDVAD-CHO (TFA) is a peptide aldehyde inhibitor used in biochemical and cell-based research to block caspase-mediated proteolysis and investigate apoptosis pathways.
- Selective caspase-2 and caspase-3 inhibition (IC50 46 nM and 15 nM).
- TFA salt, supplied as a white to off-white solid.
- High purity (99.2%).
- Molecular weight 657.59 g·mol⁻¹; formula C25H38F3N5O12.
- Recommended storage: powder at -80°C (2 years) or -20°C (1 year).
- For research use only; not for human or clinical use.
Medchemexpress LLC Ac-devd-cmk tfa | 98.6% | 664.97 | 5 MG
Ac-DEVD-CMK TFA is a selective and irreversible caspase-3 inhibitor. It significantly inhibits apoptosis induced by high levels of glucose or 3,20-dibenzoate, and can be used in various experimental approaches to inhibit apoptosis.
- Selective and irreversible caspase-3 inhibitor.
- Significantly inhibits apoptosis induced by high levels of glucose or 3,20-dibenzoate.
- Suitable for various experimental approaches to inhibit apoptosis.
- Inhibits IDB-induced apoptosis.
- Attenuates acetaminophen-induced liver injury.
- Mitigates liver damage by inhibiting GSK‐3β or caspase‐3 activity.
- Effective in inhibiting citrate-induced p21 cleavage and G2/M accumulation in human pharyngeal squamous carcinoma cell lines.
Medchemexpress LLC MANS peptide TFA | 99.9% | 2498.83 (free acid) | 1 MG
MANS peptide TFA is the TFA salt form of MANS peptide (HY-P10218). It is an inhibitor for myristoylated alanine-rich C kinase substrate (MARCKS), which competes with MARCKS in cells for membrane binding, and thus inhibits the stimulation of mucin secretion and tumor metastasis.
- Inhibits migration and invasion of lung cancer cells (CL1-0/F3, CL1-5, PC9, A549) without causing toxicity to normal cells.
- Inhibits MARCKS phosphorylation and PI3K, AKT phosphorylation, leading to downstream changes in Slug and E-cadherin expression levels.
- Prevents the loss of cell-cell adhesion and alters epithelial-mesenchymal transition (EMT) characteristics of cancer cells, thereby decreasing tumor metastasis.
- Inhibits tumor metastasis without affecting tumorigenesis in PC9 xenograft NOD/SCID mice model.
Medchemexpress LLC SsK36 TFA | 99.3% | 1771.04 (free base) | 5 MG
ssK36 TFA is a supersubstrate peptide of the histone methyltransferase (SET) domain protein 2 (SETD2). It is designed for the SETD2 protein, a specific PKMT, responsible in human cells for adding methyl groups to the 36th lysine residue of histone H3 (H3K36) to form H3K36me3. This peptide can be methylated by SETD2 at a rate more than 100 times faster than the natural substrate H3K36 and can be used to study the catalytic mechanism of PKMTs, especially substrate specificity and catalytic efficiency.
- Purity of 99.34%
- Molecular weight of 1771.04 (free base)
- Formula: C79H127N29O18.xC2HF3O2
- Appearance: Solid
- Color: White to off-white
- Sequence: Ala-Pro-Arg-Phe-Gly-Gly-Val-Lys-Arg-Pro-Asn-Arg-Tyr-Arg-Pro
- Sequence shortening: APRFGGVKRPNRYRP
- Solubility (in vitro): 1 mg/mL in H2O (requires ultrasonic)
Medchemexpress LLC PBA-1105 TFA | 99.6% | 754.83 | 5 MG
PBA-1105 TFA is an autophagy-targeting chimera (AUTOTAC) that induces p62 self-oligomerization. It selectively binds to exposed hydrophobic regions of misfolded proteins, facilitating their degradation via the autophagic pathway. This compound increases the autophagic flux of Ub-conjugated aggregates and induces autophagic degradation of misfolded proteins and aggregates at nanomolar concentrations. It is for research use only.
- Induces p62 self-oligomerization.
- Selectively binds to exposed hydrophobic regions of misfolded proteins.
- Facilitates degradation via the autophagic pathway.
- Increases the autophagic flux of Ub-conjugated aggregates.
- Induces autophagic degradation of mutant tau and misfolded proteins.
Medchemexpress LLC UNC8732 TFA | 2929304-06-1 | 99.07% | 723.74 | 1 MG
UNC8732 (TFA) is a NSD2 inhibitor and degrader.
- NSD2 inhibitor and degrader.
- For research use only.
Medchemexpress LLC M65 TFA | 99.98% | 4823.53 (free base) | 5 MG
M65 TFA is a deleted peptide of maxadilan (61 a.a.), specifically missing residues between positions 24 and 42. It functions as a specific antagonist of the PACAP type 1 receptor, inhibiting ANP secretion, and is utilized in relevant research.
- Deleted peptide of maxadilan (61 a.a.) with a deletion of residues between positions 24 and 42.
- Acts as a specific antagonist of the PACAP type 1 receptor.
- Inhibits ANP secretion.
- Completely blocks cAMP accumulation stimulated by 100 nM of VIP in rat cortical neurons at 1 μM concentration.
- Partially inhibits cAMP accumulation stimulated by 1 nM of maxadilan in rat cortical neurons at 1 μM concentration.
Medchemexpress LLC Cn2 toxin TFA (β-Mammal toxin Cn2 TFA) | 96.6% | 7588.60 (free base) | 1 MG
Cn2 toxin TFA (β-Mammal toxin Cn2 TFA) is a single-chain β-scorpion neurotoxic peptide and the main toxin found in scorpion venom. This peptide specifically targets mammalian voltage-gated sodium channels (VGSC) Nav1.6, making it a valuable tool for research in ion channel modulation and neurological diseases.
- Neurotoxic peptide
- Specifically targets mammalian voltage-gated sodium channels (VGSC) Nav1.6
- Functions as an ion channel modulator
- Useful in neurological disease research
- Acts as an inhibitor
Medchemexpress LLC Mazdutide TFA | 99.7% | 4563.06 (free base) | 5 MG
Mazdutide TFA is a long-acting synthetic oxyntomodulin analog that acts as a co-agonist of glucagon-like peptide (GLP-1R) and glucagon receptor (GCGR). It binds to both human and mouse GCGR and GLP-1R, stimulating insulin secretion from mouse islets. This compound is utilized in research for conditions such as obesity and type 2 diabetes.
- Functions as a co-agonist of GLP-1R and GCGR.
- Binds to human and mouse GCGR and GLP-1R.
- Stimulates insulin secretion from mouse islets.
- Applicable in studies for obesity and type 2 diabetes.
- Significantly reduces body weight and physical intake in diabetic mice with diet-induced obesity.